Course: General Microbiology

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Course title General Microbiology
Course code BOT/OBMSB
Organizational form of instruction Lecture + Exercise
Level of course Bachelor
Year of study not specified
Semester Summer
Number of ECTS credits 3
Language of instruction Czech
Status of course Compulsory, Compulsory-optional, Optional
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
  • Medková Jarmila, MUDr. CSc.
  • Mieslerová Barbora, doc. RNDr. Ph.D.
  • Sedlářová Michaela, doc. RNDr. Ph.D.
Course content
Lectures: The role of microorganisms in nature. Microbiology as a science: scope of the research field, its historical overview and personalities. The microbial cell structure: focused on bacteria. Nutrition and metabolism of bacteria (fermentation, respiration). Growth and cultivation of microorganisms, influence of environmental factors, compounds with antimicrobial activity and mechanisms of their effect. Genetic information, its transfer and potential for biotechnological applications. Proteosynthesis - its characteristics in bacteria. Microbial ecology, the importance if microorganisms in nutrient cycles. Mechanisms of symbiosis and pathogenesis, the interactions of microorganisms with humans, animals and plants. Bacteriophages. Taxonomy and phylogeny of Archea and Bacteria, characteristics of important microbial groups and taxa. Species of economic importance. Practicals: routine methods used for isolation, cultivation and identification of microorganisms.

Learning activities and teaching methods
Lecture, Observation, Demonstration, Projection (static, dynamic), Laboratory Work
Learning outcomes
Introduction of the microorganisms role in ecosystems, their characteristics and cultivation conditions.
After attending the course a student should be able to: - construct a timeline of significant events in the history of microbiology and human diseases, indicate outstanding personalities and their contribution to development of microbiology - select and employ proper methods for determination of microorganisms in samples from various environments (water, air, soil, plant material, medicinal samples) - select staining methods and microscopy techniques to study cell structure - modify cultivation guidelines according to biology of microorganisms and their environmental requirements - apply principles of disinfection/sterilization during growth media preparation, inoculation and cultivation to avoid overgrowth of microorganisms in axenic cultures - show interaction of microorganisms with other (micro)organisms - apply knowledge on bacterial genetics in plant biotechnology

Assessment methods and criteria
Mark, Oral exam, Written exam

Credit: 100% attendance in practicals, test, protocols Exam: extent knowledge of the lectures (test + oral exam)
Recommended literature
  • Bednář et al. (1996). Mikrobiologie lékařská.
  • Klaban V. (1999). Svět mikrobů. Hradec Králové.
  • Němec M.,Horáková D. (1993). Základy mikrobiologie. Brno.
  • Prescott, L.M., Harley, J.P., Klein, D.A. (2005). Microbiology. 6th edition. McGraw-Hill.
  • Rosypal S. (1981). Obecná bakteriologie. SNP,Praha.
  • Willey, JM, Sherwood, LM, Woolverton, CJ. (2009). Prescott´s Principles in Microbiology. McGraw-Hill.

Study plans that include the course
Faculty Study plan (Version) Branch of study Category Recommended year of study Recommended semester
Faculty of Science Botany (2015) Biology courses 1 Summer
Faculty of Science Biology and Ecology (2015) Biology courses 1 Summer
Faculty of Science Biology (1) Biology courses 3 Summer
Faculty of Physical Culture Biology (1) Biology courses 3 Summer
Faculty of Science Molecular and Cell Biology (2015) Biology courses 1 Summer
Faculty of Science Teaching Training in Biology for Secondary Schools (2015) Pedagogy, teacher training and social care 1 Summer