Course: Field Phytopathology

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Course title Field Phytopathology
Course code BOT/PFP
Organizational form of instruction Lecture + Exercise
Level of course Master
Year of study not specified
Semester Summer
Number of ECTS credits 4
Language of instruction Czech
Status of course Compulsory-optional, Optional
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
  • Lebeda Aleš, prof. Ing. DrSc.
  • Sedlářová Michaela, doc. RNDr. Ph.D.
Course content
The main aims are collecting samples in the field and documentation; determination of causal agents; methods of cultivation and inoculation; microscopical study of infection structures of fungal pathogens and pathological changes in plant anatomy; digital data processing, image analysis in phytopathology. 1. Diagnostics of plant diseases. Symptoms, microscopical methods, biochemical and molecular markers. Causal agents - focused on viruses, bacteria, oomycetes, fungi. 2. Isolation of pathogens from infected plant material. Methods of cultivation and inoculation. Collections of phytopatogenic microorganisms - Czech Republic and EU. 3. Testing of plant resistance to pathogens, or resistance of pathogens to compounds used in plant protection. 4. Pathological plant anatomy and physiology - methods of study. 5. Digital image processing, image analysis in phytopathology. 6. Automatic classification vs. trends in taxonomy of fungal pathogens. 7. Collections of phytopathogenic microorganisms in CR and EU

Learning activities and teaching methods
Dialogic Lecture (Discussion, Dialog, Brainstorming), Demonstration, Laboratory Work
Learning outcomes
The course introduces groups of phytopathogens spread in the Czech Republic.
After attending the course a student should be able to: - Compare cultivation and inoculation methods used for various groups of plant pathogens according to their integrity and life strategy - Analyse digital images in order to classify fungal pathogens - Calculate degree of pathogen sporulation, determine damage of host tissues - Compare host reaction to pathogen attac in laboratory and field conditions - Debate effects of plant protection practices on plant metabolism and crop yield

Assessment methods and criteria
Dialog, Seminar Work

1. Previous or concurrent attendance of the subjects plant physiology 2. Assay on given topic/digital data processing 3. Colloquial discussion
Recommended literature
  • Agrios, G.N. (2005). Plant pathology.. Elsevier Academic Press, 5th edition.
  • Böhm, F. X. (1936). Perspektiva. Č. Budějovice.
  • Ellis, M.B., Ellis J.P. (1997). Microfungi on land plants.. Richmond Publishing, UK.
  • Gonzales R.C., Woods R.E. (2008). Digital Image Processing.. Prentice Hall, 3rd edition.
  • Hluchý, M., Ackermann, P., Zacharda, M., Bagar, M., Jetmarová, E., Vaněk, G. (1997). Obrazový atlas chorob a škůdců ovocných dřevin a révy vinné.. Biocont laboratory, Brno.
  • Kůdela, V., Novacky, A., Fučíkovsky, L. (2002). Rostlinolékařská bakteriologie.. Academia, Praha.
  • Miranda, M., Lebeda, A. (2008). Mass Screening Techniques for Early Selection of Disease Resistance in Neglected Crops: A Critical Appraisal.. Springer Publishers, in press.
  • Rod, J., Hluchý, M., Zavadil, K., Prášil, J., Somssich, I., Zacharda, M. (2005). Obrazový atlas chorob a škůdců zeleniny střední Evropy.. Biocont laboratory, Brno.
  • Sedlářová, M., Vašutová, M., Petrželová, I. a kol. Atlas houbových organizmů (
  • Sedlářová, M., Vinter, V. Patologické změny rostlinných pletiv.
  • Šutic, D.D., Sinclair, J.B. (1991). Anatomy and physiology of diseased plants.. CRC Press, Boca Raton, Florida.

Study plans that include the course
Faculty Study plan (Version) Branch of study Category Recommended year of study Recommended semester
Faculty of Science Teaching Training in Biology for Secondary Schools (2015) Pedagogy, teacher training and social care 1 Summer
Faculty of Science Botany (2015) Biology courses 1 Summer
Faculty of Science Molecular and Cell Biology (2015) Biology courses - Summer