Course: Techniques of Plant Tissue Cultures

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Course title Techniques of Plant Tissue Cultures
Course code BOT/PGSEX
Organizational form of instruction Lecture
Level of course Doctoral
Year of study not specified
Semester Winter and summer
Number of ECTS credits 20
Language of instruction Czech, English
Status of course unspecified
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
  • Strnad Miroslav, prof. Ing. CSc., DSc.
  • Navrátilová Božena, RNDr. Ph.D.
Course content
Plant explant cultures, history and basic fundamentals, principles of work, possibilities of plant regeneration, influence of plant growth regulators, somatic variability, morphogenesis (organogenesis, embryogenesis), basic methods (meristem culture, callus culture, anther culture, protoplast culture, protoplast fusion, embryo culture, somatic embryogenesis), practically utilization of explant cultures (plant breeding, secondary metabolites, transgenosis, cryopreservation).

Learning activities and teaching methods
Dialogic Lecture (Discussion, Dialog, Brainstorming)
Learning outcomes
Training course on plant tissue cultures.
Knowledge, abilities and skills of students are in the range of examined topics.

Assessment methods and criteria
Mark, Oral exam

PhD Exam
Recommended literature
  • Callaway D. J., Callaway M. B. (2000). Breeding Ornamental Plants. Timber press, Oregon.
  • Hall R.D. (1999). Plant cell culture protocols. Totowa, New Yersey, Humana Press.
  • Lindsey K. (1992). Plant Tissue Culture. Manual.. Kluwer Academic Publishers, London.
  • Procházka S. a kolektiv. (1998). Fyziologie rostlin. Praha, Academia.
  • Smith R.H. (2000). Plant tissue culture. Techniques and experiments.. Academic Press.

Study plans that include the course
Faculty Study plan (Version) Branch of study Category Recommended year of study Recommended semester