Course: Current microscopy and image analysis

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Course title Current microscopy and image analysis
Course code BOT/SMAO
Organizational form of instruction Lecture + Exercise
Level of course Master
Year of study not specified
Semester Summer
Number of ECTS credits 4
Language of instruction Czech
Status of course Optional
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
  • Sedlářová Michaela, doc. RNDr. Ph.D.
  • Ondřej Vladan, doc. RNDr. Ph.D.
Course content
Principles of light microscopy, phase contrast, Nomarski differential contrast. Fluorescence microscopy, confocal laser scanning microscopy, techniques utilising interactions of fluorescent signals (FRAP, FRET, FLIM), correlative microscopy. Methods for fluorescent labeling of samples (immunohistochemical staining, fluorochromes for in vivo microscopy, fluorescent proteins). Image analysis - co-localization signals, measuring the intensity, size, counting objects. Softwares.

Learning activities and teaching methods
Demonstration, Projection (static, dynamic)
Learning outcomes
Students will meet new trends in the field of light microscopy, which form an integral part of most branches of biology. Software processing of acquired image data will complement the microscopic part.
After completing the course the student should: - apply the techniques of light microscopy - label samples with fluorochromes according to standard protocols - obtain and process the image data

Assessment methods and criteria
Oral exam, Written exam, Student performance

participation + practical preparation of samples and processing of image data
Recommended literature
  • Abelson J.N., Simon M.I., Conn P.M. (1999). Microscopy. Methods in Enzymology, Volume 307. Academic Press.
  • Claxton N.S., Fellers TJ., Davidson M.W. (2004). Laser Scanning Confocal Microscopy. Hibbs A.R. (2004) Confocal Microscopy for Biologists. BIOCON Melbourne.
  • Pawley J.B. (2005). Handbook of Biological Confocal Microscopy, 3rd Edition. New York.
  • Price R., Jerome J. (2010). Basic Confocal Microscopy.
  • Skalický J., Bělohlavý K., Večeřa J. (2010).
  • Svítil P., Kuchař J. (2009). Konfokální mikroskopie. na

Study plans that include the course
Faculty Study plan (Version) Branch of study Category Recommended year of study Recommended semester
Faculty of Science Botany (2015) Biology courses - Summer
Faculty of Science Teaching Training in Biology for Secondary Schools (2015) Pedagogy, teacher training and social care - Summer