Course: Basic Histochemistry

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Course title Basic Histochemistry
Course code HIE/VAB21
Organizational form of instruction Seminar
Level of course Master
Year of study not specified
Semester Summer
Number of ECTS credits 1
Language of instruction English
Status of course Optional
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Course availability The course is available to visiting students
Lecturer(s)
  • Vyhlídalová Pavla, Mgr. Ph.D.
  • Čížková Kateřina, Mgr. Ph.D.
Course content
Bases of Histochemistry and Immunohistochemistry. The course covers basic histochemical methods used for the detection of various chemical substances (e.g. lipids, iron, polysacharides and enzymes) and the principles these methods are based on. In addition, their practical use and importance for the understanding of chemical processes in human body will be discussed. Immunohistochemical techniques as a subset of histochemistry offer high specificity and sensitivity for the detection of intra- and extracellular components using a specific bond of antibody to antigen. The principle and practical application in research and diagnostics will be discussed. Upon completion, students will be equipped with knowledge needed for following subjects and particularly for pathology which takes advantage of these methods in diagnostics.

Learning activities and teaching methods
Lecture
Learning outcomes
The course covers basic histochemical methods used for the detection of various chemical substances (e.g. lipids, iron, polysacharides and enzymes) and the principles these methods are based on. In addition, their practical use and importance for the understanding of chemical processes in human body will be discussed. Immunohistochemical techniques as a subset of histochemistry offer high specificity and sensitivity for the detection of intra- and extracellular components using a specific bond of antibody to antigen. The principle and practical application in research and diagnostics will be discussed.
Upon completion, students will be equipped with knowledge needed for following subjects and particularly for pathology which takes advantage of these methods in diagnostics.
Prerequisites
Knowledge from histology.
HIE/VAB11 and HIE/VAA12

Assessment methods and criteria
Essay

Attendance at seminars is compulsory.
Recommended literature
  • Kiernan, J. (2008). Histological and Histochemical Methods: Theory and Practice. Cold Spring Harbor Laboratory Press.
  • Wick, M. R. (2008). Diagnostic Histochemistry. Cambridge University Press.


Study plans that include the course
Faculty Study plan (Version) Branch of study Category Recommended year of study Recommended semester
Faculty of Medicine and Dentistry General Medicine (2) Medical sciences 2 Summer
Faculty of Medicine and Dentistry General Medicine (2014) Medical sciences 2 Summer