Course: Experimental Methods in Plant Physiology

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Course title Experimental Methods in Plant Physiology
Course code KBF/EMFR
Organizational form of instruction Lecture
Level of course Master
Year of study not specified
Semester Winter
Number of ECTS credits 3
Language of instruction Czech
Status of course Compulsory, Compulsory-optional
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
  • Špundová Martina, RNDr. Ph.D.
Course content
1. Plant water status. Parameters of water status (RWC, water potential and its components). Transpiration (gasometric measurement and thermocamera). Water transport (gravimetry, potometry). Membrane transport. Measurement of ion leakage through membranes. 2. Photosynthetically active radiation (PAR): sources, spectra, units, measurement. PAR in canopy, LAI. Optical phenomena in leaf (reflectance, transmittance). Plant pigments (properties and functions; analytical and non-destructive measurement of pigment content). Chloroplast movement. Optical indexes used in plant physiology. Remote-sensing. 3. Study of structure of photosynthetic apparatus in higher plants (leaf, chloroplast, thylakoid membranes, pigment-protein complexes, microscopy, isolation, electrophoresis). Primary (light) reaction of photosynthesis. Oxygen evolution. Chlorophyll fluorescence (properties, fluorimeters, measurement, parameters). Phenotyping. 4. Secondary (dark) photosynthetic reactions. CO2 assimilation (CO2 diffusion, gasometric systems). Stomata (number of stomata, stomatal conductance). Growth. Dependence of photosynthetic rate on external factors (PAR, CO2, temperature).

Learning activities and teaching methods
Lecture, Monologic Lecture(Interpretation, Training)
  • Homework for Teaching - 10 hours per semester
  • Preparation for the Exam - 40 hours per semester
  • Attendace - 28 hours per semester
Learning outcomes
Knowledge of principles and use of experimental methods in plant physiology.
Knowledge Define the main ideas and conceptions of the subject, describe the main approaches of the studied topics, recall the theoretical knowledge for solution of model problems.
Knowledge in plant physiology. Basic knowledge of physics and chemistry. The subject LRR/FRP - Plant Physiology is recommended to pass.

Assessment methods and criteria
Mark, Oral exam

Passing the oral examination. Knowledge within the expent of the lecture.
Recommended literature
  • Boyer, J.S. (1995). Measuring Water Status of Plants and Soils. Academic Press, San Diego et al.
  • Campbell, G.S., Norman, J.M. (1988). An Introduction to Environmental Biophysics. Springer, New York.
  • Kramer, P.J., Boyer, J.S. (1995). Water Raelations of Plants and Soils. Academic Press.
  • Nobel, P.S. (1999). Physicochemical and Environmental Plant Physiology. Academic Press, San Diego et al.
  • Smith, J.A.C., Griffiths, H. (1993). Water Deficits. Bios Scientific Publishers, Oxford.
  • Taiz, L., Zeiger, E. (2006). Plant Physiology. Sinauer Associates, Inc., Publisher, Sunderland, Massachussets.
  • Volkov, A.G. (2006). Plant Electrophysiology. Theory and Methods.. Springer, Berlin, Heidelbnerg.
  • von Caemmerer, C. (2000). Biochemical Models of Leaf Photosynthesis. CSIRO Publishing, Collingwood, Australia.

Study plans that include the course
Faculty Study plan (Version) Branch of study Category Recommended year of study Recommended semester
Faculty of Science Biophysics (2015) Physics courses 1 Winter
Faculty of Science Molecular Biophysics (2015) Physics courses 1 Winter
Faculty of Science Experimental Biology of Plants (1) Biology courses 1 Winter