Course: Physical Properties of Nucleic Acids

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Course title Physical Properties of Nucleic Acids
Course code KBF/FVNK
Organizational form of instruction Lecture
Level of course Master
Year of study not specified
Semester Winter
Number of ECTS credits 3
Language of instruction Czech
Status of course Compulsory-optional, Optional
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
  • Brabec Viktor, prof. RNDr. DrSc.
  • Kašpárková Jana, prof. RNDr. Ph.D.
Course content
1. Structure and fundamental properties of DNA and RNA. 2. History of discovery of DNA structure. 3. Components, conformation, A-, B-, and Z-form. 4. Forces stabilizing double helix. 5. Higher organization of DNA, chromosomes, nucleosomes, chromatin. 6. Forms of t-RNA, ribosomes. 7. Interactions of DNA with ions, hydrodynamic properties, viscosity. 8. Sedimentation, diffusion, osmosis. 9. Absorption spectra, hypochromicity, thermal stability and thermodynamics of nucleic acids. 10. Thermodynamics of nucleic acids. 11. Optical anisotropy and activity, fluorescence. 12. Electric and magnetic properties of DNA and RNA. 13. Electrophoresis of nucleic acids. 14. Electrochemistry of nucleic acids. 15. Artificial DNA, expanded DNA.

Learning activities and teaching methods
  • Attendace - 26 hours per semester
  • Homework for Teaching - 13 hours per semester
  • Preparation for the Exam - 70 hours per semester
Learning outcomes
Structure and basic properties od DNA and RNA
Knowledge Define the main ideas and conceptions of the subject, describe the main approaches of the studied topics, recall the theoretical knowledge for solution of model problems.

Assessment methods and criteria
Student performance

Knowledge within the scope of the course topics (colloquium)
Recommended literature
  • Brabec, V., Kleinwachter, V., Vetterl, V. (1997). Struktura, chemická reaktivita a elektromagnetické vlastnosti nukleových kyselin. In Biochemistry and Biomacromolecules. Basel: Birghauser Verlag, s. 1-104.
  • Fojta, M., Vetterl, V., Tomschik, M., Jelen, F., Nielsen, P. (1997). Adsorption of peptide nucleic acid and DNA decamers at electrically charged surfaces. Biophysical Journal, Amsterdam: Elsevier Scientific, 72, s. 2285-2293.
  • Jelen, F., Vetterl, V., Běluša, P., Hasoň, S. Adsorptive Stripping Analysis of DNA with Admittance Detection. Elecztroanalysis, Weinheim: Willey-VCH Verlag GmbH, 12, 12, s. 987-992.
  • Vetterl, V., Papadopoulos, N., Dražan, V., Strašák, L., Hasoň, S., Dvořák, J. Nucleic acid sensing by impedance measurements. Electrochimica Acta, Amsterdam: Elsevier Science, 45, 18, s. 2961-2971.

Study plans that include the course
Faculty Study plan (Version) Branch of study Category Recommended year of study Recommended semester
Faculty of Science Molecular Biophysics (2015) Physics courses 2 Winter