Course: Optical Spectroscopies 2

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Course title Optical Spectroscopies 2
Course code KBF/OSP2
Organizational form of instruction Lecture + Exercise
Level of course Bachelor
Year of study not specified
Semester Summer
Number of ECTS credits 5
Language of instruction Czech
Status of course Compulsory, Compulsory-optional
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
  • Pospíšil Pavel, doc. RNDr. Ph.D.
Course content
1. Polarization spectroscopy Definition and classification of polarized light. Theory of circular dichroism, chiral molecules, optically active chromophores, optical rotation dispersion, circular dichroism, ORD spectra, CD spectra, application of ORD and CD spectroscopy in biology. 2. Photoacoustic spectroscopy Principle of photoacoustic spectroscopy, frequency modulation of photoacoustic signal, thermal diffusion length, classification of photoacoustic effect, direct and indirect photoacoustic methods, advanced methods of photoacoustic spectroscopy, photothermal deflection spectroscopy, time-resolved thermal lensing, photoacoustic microscopy, comparison of absorption and photoacoustic spectroscopy, photoacoustic spectrometer (filters, sample, photoacoustic chamber, detector), application of photoacoustic spectroscopy in biology. 3. Elastic and quasielastic light scattering Definition and classification of elastic light scattering, Rayleigh scattering, Mie scattering, theory of the Rayleigh and Mie scattering. Definition and classification of quasielastic light scattering, autocorrelation function, particle size determination, Tyndal scattering, turbidimetry, nephelometry, experimental setup, application of light scattering in biology. 4. Light diffraction, reflection and refraction spectroscopy Light diffraction, theory of light diffraction, methods, experimental setup and application in biology. Light reflection, Fresnel equations, reflection from a flat surface, multiple reflection, diffuse reflection. Light refraction, refractometer. 5. Vibrational absorption spectroscopy Classical and quantum theory of small vibrations, active vibrations, IR spectra, number and peak position in IR spectrum, IR spectral region, typical vibrational frequency in mid-IR region, classical IR spectroscopy, FTIR spectroscopy, polarized IR spectroscopy, two-dimensional IR spectroscopy, dispersive IR spectrometer, FTIR spectrometer, application of IR spectroscopy in biology. 6. Rotation absorption spectroscopy Classical and quantum theory of molecular rotation, selection rule, dynamic rotation spectroscopy, Fourier-transformed rotation spectroscopy, application of rotation spectroscopy in biology. 7. Raman scattering Principle of Raman scattering, Stokes and Anti-Stokes scattering, selection rule, classification of Raman scattering, Raman spectra, comparison of IR and Raman spectra, resonance-enhanced Raman scattering, surface-enhanced Raman scattering, stimulated Raman scattering, coherent Raman scattering, non-linear Raman scattering, Raman spectrometer, CARS spectrometer, SERS, application of Raman spectroscopy in biology.

Learning activities and teaching methods
Monologic Lecture(Interpretation, Training)
  • Preparation for the Exam - 30 hours per semester
  • Preparation for the Course Credit - 10 hours per semester
  • Attendace - 36 hours per semester
Learning outcomes
Introduction to theory, spectra, methods, experimental instrumentation and biological application of advanced optical spectroscopy.
Understanding of theory, spectra, methods, experimental instrumentation and biological application of advanced optical spectroscopy.

Assessment methods and criteria
Oral exam

Passing written test and oral examination.
Recommended literature
  • Duxbury, G. (1999). Infrared Vibration-Rotation Spectroscopy: From Free Radicals to the Infrared Sky. John Wiley & Sons.
  • Prosser, V. a kol. (1989). Experimentální metody biofyziky. Academia Praha.
  • Serdyuk, I. N., Nathan R., Zaccai, J. (2007). Methods in Molecular Biophysics : Structure, Dynamics, Function. Cambridge.
  • Žaloudek, F. (1986). Experimentální metody biofyziky III. (skripta). UP Olomouc.

Study plans that include the course
Faculty Study plan (Version) Branch of study Category Recommended year of study Recommended semester
Faculty of Science Experimental Biology (2016) Biology courses 3 Summer
Faculty of Science Biophysics (2015) Physics courses 3 Summer
Faculty of Science Molecular Biophysics (2015) Physics courses 3 Summer