Course: Structure, Properties, and Function of Nucleic Acids

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Course title Structure, Properties, and Function of Nucleic Acids
Course code KBF/PGSNK
Organizational form of instruction Lecture
Level of course Doctoral
Year of study not specified
Semester Winter and summer
Number of ECTS credits 20
Language of instruction Czech, English
Status of course unspecified
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
  • Brabec Viktor, prof. RNDr. DrSc.
Course content
DNA conformation. A-, B-, Z- forms. Twist, tilt. DNA curvature. Helix-coil transition, forces stabilizing double helix. Higher organization of DNA, supercoiled DNA. Condensed forms. Chromosomes, nucleosomes, chromatin. Interactions of DNA with proteins. Interaction of DNA with ions. Protonation, pK, metal ions, electrostatic shielding. Structure and properties of RNA. Double-helical RNA. A-, Z- forms of RNA. Higher organization of RNA, t-RNA, ribosomes. Hydrodynamic and spectral properties of nucleic acids. Viscosity, sedimentation, diffusion. Osmosis. Electron vibration absorption spectra, hypochromicity, melting temperature. Spectroscopy of polarized light, ORD and CD. Genetic information. Replication, transcription, translation. Genetic code. Codon. RNA-polymerases. Viruses. Retroviruses. Reverse transcription. Mutations. Recombination. Oncogens.

Learning activities and teaching methods
Lecture, Work with Text (with Book, Textbook)
  • Preparation for the Exam - 70 hours per semester
  • Homework for Teaching - 30 hours per semester
Learning outcomes
DNA conformations. Structure and properties of RNA. Hydrodynamic and spectral properties of nucleic acids.
Evaluation Evaluate the particular methods and principles, explain the aspects and results concerning the given issue, integrate the knowledge, predict the solutions, evaluate the results and outcomes.
Basic knowledge of biochemistry, molecular biophysics and molecular biology.

Assessment methods and criteria
Oral exam

Knowledge within the scope of the course topics (examination)
Recommended literature
  • Lenaz G., Milazzo D. (ed.). (1997). Bioelectrochemistry of Biomacromolecules. Birkhauser Verlag, Basel, Boston, Berlin.
  • Rosypal S. (2000). Úvod do molekulární biologie, I, II, III. Brno.

Study plans that include the course
Faculty Study plan (Version) Branch of study Category Recommended year of study Recommended semester