Course: Advanced Practicum from Experimental Methods of Plant Physiology

« Back
Course title Advanced Practicum from Experimental Methods of Plant Physiology
Course code KBF/PPEMF
Organizational form of instruction Exercise
Level of course Master
Year of study not specified
Semester Winter
Number of ECTS credits 3
Language of instruction Czech
Status of course Optional
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Lecturer(s)
  • Kubala Martin, doc. RNDr. Ph.D.
  • Lazár Dušan, doc. RNDr. Ph.D.
Course content
- Instruction for security of work, formation of working groups - Preparation of chloroplast suspension - Emission and excitation spectra of chlorophyll - explanation of the method, meaning of evaluated parameters, measurement of the spectra by spectrofluorometer Hitachi F4500 in room temperature and 77 K using control and stressed samples - Slow fluorescence induction and fluorescence quenching - explanation of the method, meaning of evaluated parameters, measurement of the induction and quenching by fluorometer PAM 2000 using control and stressed samples - Very fast fluorescence induction - explanation of the method, meaning of evaluated parameters, measurement of the induction by fluorometer PEA using control and stressed samples - Light induced changes in emission spectra - explanation of the method, meaning of evaluated parameters, measurement of the spectra at room temperature by spectrophotometer Olis using control and stressed samples - Synchronous detection of very fast fluorescence induction and A820 signal - explanation of the method, meaning of evaluated parameters, synchronous measurement of the induction and A820 signal by fluorometer Dual PAM 100 using control and stressed samples - Very fast detection of P515 signal - explanation of the method, meaning of evaluated parameters, measurement of the P515 signal by fluorometer Dual PAM 100 using control and stressed samples - Fluorescence imaging - explanation of the method, meaning of evaluated parameters, measurement of the imaging by fluorometer FluorCam using control and stressed samples - Fluorescence temperature curve - explanation of the method, meaning of evaluated parameters, measurement of the curve by laboratory-made apparatus using control and stressed samples

Learning activities and teaching methods
Laboratory Work
Learning outcomes
Emission and excitation spectra of chlorophyll. Slow fluorescence induction and fluorescence quenching.
Show an ability to apply knowledge and principles for solution of particular problems in the concrete situations.
Prerequisites
unspecified

Assessment methods and criteria
Student performance

Student uses manuals to practive excercises made by the members of Department of Biophysics.
Recommended literature
  • manuály ke použitým komerčním přístrojům.
  • vybrané časopisecké publikace.


Study plans that include the course
Faculty Study plan (Version) Branch of study Category Recommended year of study Recommended semester
Faculty of Science Experimental Biology of Plants (1) Biology courses 2 Winter