Course: Practicum from Spectroscopic Methods

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Course title Practicum from Spectroscopic Methods
Course code KBF/PSM
Organizational form of instruction Exercise
Level of course Master
Year of study not specified
Semester Summer
Number of ECTS credits 4
Language of instruction Czech
Status of course Compulsory, Optional
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
  • Pospíšil Pavel, doc. RNDr. Ph.D.
Course content
Instructions on safety, splitting into groups Single-beam spectrophotometry Double-beam spectrophotometry Preparation of chloroplast suspension Fluorescence I Hill reactions Extinction of fluorescence Fluorescence II FTC and fluorescent induction Measurement of water potential Fluorescent microscopy Laser flow cytometry I Laser flow cytometry II Evaluation of class duties Alternative laboratory tasks: TLC and reflective spectrophotometry, double-beam spectrophotometry II

Learning activities and teaching methods
Laboratory Work
  • Attendace - 36 hours per semester
  • Homework for Teaching - 10 hours per semester
Learning outcomes
Spectrophotometry . Fluorescence .Measurement of water potential .Laser flow cytometry .
Application Show an ability to apply knowledge and principles for solution of particular problems in the concrete situations.

Assessment methods and criteria
Student performance

Recommended literature
  • Englich, J. a kol. (1983). Experimentální metody biofyziky II - metody magnetické rezonance. UK Praha.
  • Prosser, V. a kol. (1989). Experimentální metody biofyziky. Academia Praha.
  • Rákoš, M. (1988). Rádio-spektroskópické metódy. Alfa Bratislava.
  • Slichter, C.P. (1992). Principles of Magnetic Resonance (3rd edition). Springer-Verlag, New York, Berlin, Heidelberg.

Study plans that include the course
Faculty Study plan (Version) Branch of study Category Recommended year of study Recommended semester
Faculty of Science Experimental Biology (2015) Biology courses - Summer
Faculty of Science Experimental Biology of Plants (1) Biology courses 1 Summer
Faculty of Science Molecular Biophysics (2015) Physics courses 1 Summer
Faculty of Science Biophysics (2015) Physics courses 1 Summer