Course: Resonance Spectroscopies

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Course title Resonance Spectroscopies
Course code KBF/RSP
Organizational form of instruction Lecture + Exercise
Level of course Master
Year of study not specified
Semester Winter
Number of ECTS credits 5
Language of instruction Czech
Status of course Compulsory, Optional
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Lecturer(s)
  • Pospíšil Pavel, doc. RNDr. Ph.D.
Course content
1. Introduction in resonance spectroscopy. Principle of resonance spectroscopy. Quantum-mechanistic characterization of atomic nucleus and electrons. 2. Nuclear magnetic resonance (NMR). NMR theory, static, time-dependent a pulsed magnetic field, classical theory (precession, nutation, relaxation process), quantum theory (splitting of energy levels, Zeeman effect), phenomenological theory (magnetization, response function, continuous and pulsed solution of Bloch equations, free induction decay, spin echo). NMR spectra, number, intensity, position and splitting of NMR signal. NMR methods, one-dimensional NMR, 1H-NMR (NOED, ID NMR), 13C-NMR (broad band, off resonance, spin-echo), multi-dimensional NMR, homonuclear (COSY, MQF-COSY, NOESY), heteronuclear (HETCOR, COLOC, HSQC, HMQC, HMBC), NMR imaging. Experimental setup (magnet, frequency generator, detector), continuous and pulsed NMR spectrometer. Application of NMR in biology. 3. Electron paramagnetic resonance (EPR). EPR theory, splitting of energy levels, Zeeman effect, free radical, transitions metal, spin-orbital interaction (g-factor, anisotropy), spin-spin interaction (electron-nucleus), spin-spin interaction (electron-electron), comparison of EPR a NMR. EPR spectra, number, intensity, position and splitting of EPR signal. EPR methods, continuous EPR (EPR spin-trapping, EPR labeling, cw ENDOR/ELDOR), pulsed EPR (FT-EPR, pulsed ENDOR/ELDOR, ESEEM). Experimental setup (magnet, klystrons, resonator, cryogenic technique). Application of EPR v biology. 4. Mössbauer spectroscopy. Theory, Mössbauer effect, emission and absorption of gamma ray, nucleus free to recoil or bound in crystal lattice. Mössbauer spectra, hyperfine interaction (monopole, quadrupole, magnetic dipole), qualitative and quantitative analysis. Methods of Mössbauer spectroscopy. Experimental setup, Mössbauer spectrometer. Application of Mössbauer spectroscopy in biology.

Learning activities and teaching methods
Monologic Lecture(Interpretation, Training)
  • Attendace - 3 hours per semester
  • Preparation for the Exam - 24 hours per semester
Learning outcomes
Introduction to theory, spectra, methods, experimental instrumentation and biological application of nuclear magnetic resonance, electron paramagnetic resonance and Mössbauer spectroscopy.
Understanding of theory, spectra, methods, experimental instrumentation and biological application of nuclear magnetic resonance, electron paramagnetic resonance and Mössbauer spectroscopy.
Prerequisites
unspecified

Assessment methods and criteria
Oral exam

Passing written test and oral examination.
Recommended literature
  • Amesz, J., Hoff, A. J. (1996). Biophysical Techniques in Photosynthesis. Kluwer Academic Publishers, Dordrecht, Boston, London.
  • Englich, J. a kol. (1983). Experimentální metody biofyziky II - metody magnetické rezonance. UK Praha.
  • Hemminga, Marcus A., Berliner, Lawrence, J. (2007). ESR Spectroscopy in Membrane Biophysics. Series: Biological Magnetic Resonance , Vol. 27.
  • Mašláň, M. (1993). Mossbauerova spektroskopie. UP Olomouc.
  • Prosser, V. a kol. (1989). Experimentální metody biofyziky. Academia Praha.


Study plans that include the course
Faculty Study plan (Version) Branch of study Category Recommended year of study Recommended semester
Faculty of Science Biophysics (2015) Physics courses 1 Winter
Faculty of Science Experimental Biology (2015) Biology courses - Winter
Faculty of Science Molecular Biophysics (2015) Physics courses 1 Winter
Faculty of Science Experimental Biology of Plants (1) Biology courses 1 Winter