Course: Structure, Function and Interaction of Biomacromolecules

« Back
Course title Structure, Function and Interaction of Biomacromolecules
Course code KBF/STBM
Organizational form of instruction Lecture
Level of course Bachelor
Year of study not specified
Semester Summer
Number of ECTS credits 3
Language of instruction Czech
Status of course Compulsory, Compulsory-optional
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Lecturer(s)
  • Brabec Viktor, prof. RNDr. DrSc.
Course content
1. Introduction: Definition of molecular biophysics. Intermolecular forces. Thermodynamics of transitions in biological systems - a brief survey. 2. Structure and function of nucleic acids: Structure of monomeric components of nucleic acids. Primary and secondary structure of DNA. Stability of DNA. Circular DNA. 3. Unusual structures. Nucleases. 4. Structure and properties of chromosome. 5. DNA replication. 6. "RNA world". Types of RNA and structure. Transcription and translation. 7. Catalytic activity of nucleic acids - ribozymes a deoxyribozymes. Molecular evolution and "RNA world". Aptamers. 8. Application of electrochemistry for studies of nucleic acids structure. 9. Structure and function of proteins: Function of proteins. Composition of proteins, structure and properties of amino acids, configuration of peptide bond. Primary, secondary, tertiary structure of proteins, forces stabilizing protein structures, quaternary structure of proteins. 10. DNA binding proteins, their secondary structures. Effect of temperature and pH on protein structure. Chaperons, chaperonins. 11. Methods for studies of protein structures. Determination of sequence, molecular mass and methods for identification of proteins. 12. Application of NMR spectrometry for studies of biomacromolecules. 13. Structure and function of other biopolymers: Saccharides - function of saccharides, types of saccharides. Monosaccharides - structure, conformation, optical activity. Polysaccharides - conformation of glycosidic bond. Glycoproteins. 14. Lipids - composition, relationship between structure and physical properties. Composite lipids, sterols. Biophysical properties of lipids of biological significance. 15. Molecular biophysics of cellular membranes: Structure of membranes. Membrane proteins, structure and function. Passive and active transport, diffusion and osmosis. Na+/K+ pump. Ionic channels. Membrane potential and membrane voltage. Membrane and Donnan equilibrium. Action potential and transmission of excitement on membranes and neurotransmission. 16. Glycobiophysics. 17. Methods of separation of biomacromolecules for structural studies.

Learning activities and teaching methods
Lecture
  • Attendace - 26 hours per semester
  • Preparation for the Exam - 80 hours per semester
  • Homework for Teaching - 10 hours per semester
Learning outcomes
Structure and function of nucleic acids, amino acids. Structure and function of other biomolecules. Molecular biophysics of cellular membranes.
Knowledge Define the main ideas and conceptions of the subject, describe the main approaches of the studied topics, recall the theoretical knowledge for solution of model problems.
Prerequisites
Basic knowledge of biochemistry.

Assessment methods and criteria
Mark

Knowledge within the scope of the course topics (examination)
Recommended literature
  • Alberts, B., Bray, D., Johnson, A., Lewis, J., Raff, M., Roberts, K., Walter, P. (2000). Základy buněčné biologie. Espero Publishing, Ústí nad Labem.
  • Bergethon, P.R. (1998). The Physical Basis of Biochemistry. The Foundations of Molecular Biophysics. Springer.
  • Kodíček, M., Karpenko, V. (2000). Biofyzikální chemie. Academia Praha.
  • Tuszynski, J.A., Kurzynski, M. (2003). Introduction to Molecular Biophysics. CRC Press, Washington, DC.


Study plans that include the course
Faculty Study plan (Version) Branch of study Category Recommended year of study Recommended semester
Faculty of Science Molecular Biophysics (2015) Physics courses 2 Summer
Faculty of Science Experimental Biology (2015) Biology courses - Summer