Course: Advanced Microscopic Methods

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Course title Advanced Microscopic Methods
Course code KEF/MMM
Organizational form of instruction Lecture
Level of course Master
Year of study not specified
Semester Summer
Number of ECTS credits 3
Language of instruction Czech
Status of course Compulsory, Compulsory-optional, Optional
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
  • Kubínek Roman, doc. RNDr. CSc.
Course content
Light microscopy - method of phase contrast, UV and IR microscopy, fluorescent microscopy, polarization microscopy, interference microscopy (Nomarski interference contrastm Hoffman modulation contrast), confocal laser microscopy, optical scanning near-field microscopy Electron microscopy - transmission electron microscopy, scanning electron microscopy, low-voltage electron microscopy, electron microscopy with high resolution, electron microscopy with optional vacuum (biological application) Scanning probe microscopy - scanning tunneling microscopy, atomic force microscopy, magnetic force microscopy, electrostatic force microscopy, lateral force microscopy, scanning capacity microscopy, scanning temperature microscopy, scanning optical near-field microscopy, methods related to the group of methods of scanning probe microscopy

Learning activities and teaching methods
  • Attendace - 26 hours per semester
Learning outcomes
Overwiev of various microsopy methods.
The course focused on gaining the ability to understand the problem. Explain the nature and ability to interpret data, to distinguish and classify of microscopic methods.
Basic cource of physics

Assessment methods and criteria
Student performance

Knowledge of the topics, ability to discuss on the topics in wider contexts
Recommended literature
  • Kubínek, R. (1986). Experimentální metody biofyziky-rastrovací elektronová mikroskopie.. UP Olomouc.
  • Kubínek, R., Mašláň, M., Vůjtek, M. (2002). Mikroskopie skenující sondou. UP Olomouc.
  • Kubínek, R. Materials in PDF form. (available online at
  • Murphy, D.B. (2001). Fundamentals of Light Microscopy and Electronic Imagine. Wiley.
  • Reimer, L. (1998). Scanning Electron Microscopy.Physics of Image Formation and Microanalysis.. Springer.
  • Vůjtek, M., Kubínek, R., & Mašláň, M. (2012). Nanoskopie. V Olomouci: Univerzita Palackého.

Study plans that include the course
Faculty Study plan (Version) Branch of study Category Recommended year of study Recommended semester
Faculty of Science Nanotechnology (1) Special and interdisciplinary fields 3 Summer
Faculty of Science Applied Physics (1) Physics courses 3 Summer
Faculty of Science Digital and Instrument Optics (1) Physics courses 1 Summer
Faculty of Science Biophysics (2015) Physics courses 3 Summer
Faculty of Science Optics and Optoelectronics (2015) Physics courses 1 Summer
Faculty of Science Physics (2015) Physics courses 3 Summer
Faculty of Science Material Chemistry (2015) Chemistry courses 1 Summer
Faculty of Science Instrument Physics (1) Physics courses 2 Summer